Study of the best conditions for producing Glucose isomerase from Streptomyces spp.

Abstract

Streptomyces bacteria were isolated from the soil of a field of maize, the isolates were diagnosed morphologically, physiologically and biologically according to the International Streptomyces Project (ISP), and isolates were screened to evaluate their ability to produce glucose isomerase enzyme using casein starch agar medium (CSA) based on the growth density as an indicator of the consumption of xylose sugar. Factors affecting the production of glucose isomerase enzyme from Streptomyces spp. bacteria were studied, where the best incubation time was 96 hours, the maximum enzymatic activity was (1066.67) units / liter and the specific activity was (138) units / g, and the best bacterial growth and the highest productivity of the enzyme was (1010) units / liter and efficacy The specificity is (162.64) units / g at an incubation temperature of 30 ° C, The highest production of the enzyme was (1066.67) units/l and the highest specific activity (151.3) units/g at pH7, and it was found that replacing xylose sugar with other sugars could not give the enzyme more productivity than the xylose sugar. Where xylose sugar gave the highest enzymatic activity that reached (1066.67) units/l and specific activity (140.17) units/g, and when studying the optimal conditions for converting glucose into fructose at different temperatures and at different times, the temperature of 70 ° C for 60 minutes managed to give the highest concentration of fructose .The highest enzymatic activity reached (826.67) units/l, and the highest specific activity reached (115.62) units/g, resulting from a biomass weighing (7.15)g.